Cytotoxicity Evaluation of Bacteria Extract Isolated from Indonesia Sheep Manures and Fungal Fruiting Body

  • Leonny Yulita Hartiadi Lecturer
  • Immaculata Titis Winiati
  • samirah - -
  • Cristina Gomez
Keywords: cytotoxicity, anticancer, Aneurinibacillus, Ochrobactrum


Introduction: Cancer is one of the leading cause of death worldwide. Chemotherapy remains the most widely used approach to treat cancer. However, due to the lack of specificity, drug resistance, and undesirable side effects, the discovery of novel anticancer drugs is still a priority in cancer research. Bacteria are one of the major contributors for anticancer compounds as bacteria are able to produce secondary metabolites which may have cytotoxic activity. Method: A literature study was conducted to identify the isolates in i3L-USAID bacteria database that were potential candidates for novel anticancer drugs. The selected bacteria were cultured and their secondary metabolites were extracted. Cytotoxic activity of the extracts was assessed through morphological observation and MTT assay on HeLa and 3T3 fibroblast cell lines. Results: Aneurinibacillus sp. and Ochrobactrum sp. were selected. Ochrobactrum sp. extract significantly suppressed the growth of HeLa cells at 1000 µg/ml (p < 0.05) while it did not have any effect on 3T3 cells. Despite able to suppress the HeLa cell proliferation, Aneurinibacillus sp. extract significantly enhanced the growth of 3T3 fibroblast at the 1000 µg/ml (p < 0.05). The cytotoxicity activity was corroborated by cell death observed on HeLa cells treated with high concentrations of the extracts Conclusion: Secondary metabolites from Aneurinibacillus sp. and Ochrobactrum sp. could only exert cytotoxic effect at high concentration which is unfavourable for anticancer compounds. Although the extracts resulted in a reduction of HeLa cells proliferation, the extracts did not inhibit proliferation of 3T3, suggesting selectivity for non-tumorigenic over tumorigenic cells.


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Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R., Torre, L., Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal For Clinicians, 68(6), 394-424.doi: 10.3322/caac.21492

Demain, A. L., Vaishnav, P. (2010). Natural products for cancer chemotherapy. Microbial Biotechnology, 4(6), 687–699.doi:10.1111/j.1751-7915.2010.00221.x

Desriac, F., Jégou, C., Balnois, E., Brillet, B., Le Chevalier, P., & Fleury, Y. (2013). Antimicrobial peptides from marine proteobacteria. Marine drugs, 11(10), 3632-60. doi:10.3390/md11103632
Favia G, Mariggio MA, Maiorano E, Cassano A, Capodiferro S, Ribatti D. (2008) Accelerated wound healing oforal soft tissues and angiogenic effect induced by a pool of aminoacids combined to sodium hyaluronate (AMINOGAM). J BioI Regul Homeost Agents; 22:109-16.
Gause, G. F., and M. G. Brazhnikova. 1944. Gramicidin S and its use in the treatment of infected wounds. Nature 154:703
Karpiński, T., Adamczak, A. (2018). Anticancer Activity of Bacterial Proteins and Peptides. Pharmaceutics, 10(2), 54.doi: 10.3390/pharmaceutics10020054
Lee, K. C., Kim, K. K., Eom, M. K., Kim, J. S., Kim, D. S., Ko, S. H., & Lee, J. S. (2014). Aneurinibacillus soli sp. nov., isolated from mountain soil. International journal of systematic and evolutionary microbiology, 64(11), 3792-3797.
Mariggio M, Cassano A, Vinella A, Vincenti A, Fumarulo R, et. al. (2009). Enhancement of Fibroblast Proliferation, Collagen Biosynthesis and Production of Growth Factors as a Result of combining Sodium Hyaluronate and aminoacids. International Journal of Immunopathology and Pharmacology. 2009 vol: 22 (2) pp: 485-492
Miller, K. D., Siegel, R. L., Lin, C. C., Mariotto, A. B., Kramer, J. L., Rowland, J. H., … Jemal, A. (2016). Cancer treatment and survivorship statistics. CA: A Cancer Journal for Clinicians, 66(4), 271–289. doi:10.3322/caac.21349

National Cancer Institute. (2017). Types of Cancer Treatment.

Raimundo, I., Silva, S. G., Costa, R., & Keller-Costa, T. (2018). Bioactive Secondary Metabolites from Octocoral-Associated Microbes-New Chances for Blue Growth. Marine drugs, 16(12), 485. doi:10.3390/md16120485
Thirumurugan, D., Cholarajan, A., Raja, S. S. S., Vijayakumar, R. (2018). An Introductory Chapter: Secondary Metabolites. Secondary Metabolites - Sources and Applications.doi:10.5772/intechopen.79766

Vijayarathna, S., & Sasidharan, S. (2012). Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pacific Journal of Tropical Biomedicine, 2(10), 826-829. doi:10.1016/S2221-1691(12)60237-8

Zu, L., Xiong, J., Li, G., Fang, Y., & An, T. (2014). Concurrent degradation of tetrabromobisphenol A by Ochrobactrum sp. T under aerobic condition and estrogenic transition during these processes. Ecotoxicology and environmental safety, 104, 220-225.