Comparison of Antimicrobial Activity between Ethanolic Extract and Essential Oil of Ginger (Zingiber officinale) against Food-borne Bacteria
Abstract
Background: Ginger (Zingiber officinale) is one of the most well-known spices with antimicrobial activity. However, different extraction methods of ginger will result in different antimicrobial properties due to the various substances extracted. This study aimed to compare antimicrobial activity between ethanolic extract and essential oil of ginger against food-borne bacteria grown in 2.1% Mueller Hinton agar. Methods: Fresh ginger rhizomes were extracted either by using vapor distillation method or maceration using ethanol to obtain ginger essential oils (GEO) and ginger ethanolic extract (GEE), respectively. Ethanolic extract and essential oil of ginger at different concentrations were then tested for their antimicrobial activity using disk diffusion method against Escherichia coli, Bacillus subtilis, Salmonella typhi, and Staphylococcus aureus. Tetracycline was also used as a standard of antibacterial agent. Results: Ginger essential oil in 25%, 50%, and 100% showed significant growth inhibition of four types of bacteria compared to ginger ethanolic extract. This antimicrobial effect of ginger essential oil was shown to be dose-dependent. However, it has been demonstrated that ginger ethanolic extract 50% and 100% has a stronger antimicrobial effect against B.subtilis. Conclusion: Ginger essential oil and ethanolic extract showed different degree of antibacterial activity against food-borne bacteria due to compound contained within respective extracts, with a higher degree of activity found in ginger essential oil. These may show that different ginger extract may have different antibacterial activity.
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References
De Silva, B., Jung, W., Hossain, S., Wimalasena, S., Pathirana, H., & Heo, G. (2017). Antimicrobial property of lemongrass (Cymbopogon citratus) oil against pathogenic bacteria isolated from pet turtles. Laboratory Animal Research, 33(2), 84. doi: 10.5625/lar.2017.33.2.84
Grace, U., Sankari, M., & Gopi. (2017). Antimicrobial Activity of Ethanolic Extract of Zingiber Officinale – An in vitro Study. Journal Of Pharmaceutical Science And Research, 9(9).
Grzanna, R., Lindmark, L., & Frondoza, C. (2005). Ginger—An Herbal Medicinal Product with Broad Anti-Inflammatory Actions. Journal Of Medicinal Food, 8(2), 125-132. doi: 10.1089/jmf.2005.8.125
Habsah, M., Amran, M., Mackeen, M., Lajis, N., Kikuzaki, H., & Nakatani, N. et al. (2000). Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. Journal Of Ethnopharmacology, 72(3), 403-410. doi: 10.1016/s0378-8741(00)00223-3
Hammer, K., Carson, C., & Riley, T. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal Of Applied Microbiology, 86(6).
Havelaar, A. H., Kirk, M. D., Torgerson, P. R., Gibb, H. J., Hald, T., Lake, R. J., ... & Speybroeck, N. (2015). World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010. PLoS medicine, 12(12), e1001923.
Hiserodt, R., Franzblau, S., & Rosen, R. (1998). Isolation of 6-, 8-, and 10-Gingerol from Ginger Rhizome by HPLC and Preliminary Evaluation of Inhibition ofMycobacteriumaviumandMycobacteriumtuberculosis. Journal Of Agricultural And Food Chemistry, 46(7), 2504-2508. doi: 10.1021/jf970948l
Kementerian Kesehatan RI, 2016, Profil Kesehatan Indonesia Tahun 2015, Jakarta.
Park, M., Bae, J., & Lee, D. (2008). Antibacterial activity of [10]-gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria. Phytotherapy Research, 22(11), 1446-1449. doi: 10.1002/ptr.2473
Rahmani, A., Shabrmi, F., & Aly, S. (2014). Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. International Journal Of Physiology, Pathophysiology, And Pharmacology, 6(2).
Salmon, C. N., Bailey-Shaw, Y. A., Hibbert, S., Green, C., Smith, A. M., & Williams, L. A. (2012). Characterisation of cultivars of Jamaican ginger (Zingiber officinale Roscoe) by HPTLC and HPLC. Food chemistry, 131(4), 1517-1522.
World Health Organization. (2015). WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015 (No. 9789241565165). World Health Organization.
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