Bioprocessing of mAb (Monoclonal Antibodies) using Chinese Hamster Ovary (CHO) Cells: A Review

  • Richelle Bertly Josefano Institut Bio Scientia Internasional Indonesia
  • Aivyanca A Institut Bio Scientia Internasional Indonesia
  • Charlivo Mikaichi Dungus Institut Bio Scientia Internasional Indonesia
  • Jason Jonathan Hamid Institut Bio Scientia Internasional Indonesia
  • Katherine Katherine Institut Bio Scientia Internasional Indonesia
Keywords: Monoclonal antibodies, Bioprocessing process, Chinese hamster ovary (CHO) cells, Upstream prcoess, Downstream process


Monoclonal antibodies (mAb) are known to be able to be produced from Chinese Hamster Ovary (CHO) cells. It has become increasingly important throughout the years due to its efficiency and revolutionary treatment for various diseases such as cancer, autoimmune diseases, and so forth. This review examines several upstream and downstream processes involved in the production of mAb including CHO cell maintenance, cellular engineering of CHO, transfection of plasmids into CHO cells, clonal selection and screening, culture of rCHO cells, cell harvesting, purification, and polishing. Several challenges of the bioprocessing process include a lack of large up scaling for industrial purposes, high capital costs, as well as productivity inefficiencies. However, a solution proposed is for biopharmaceutical companies to research further into the downstream processing for a continuous, efficient, and productive process.


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Author Biographies

Richelle Bertly Josefano, Institut Bio Scientia Internasional Indonesia

Department of Biotechnology, Institut Bio Scientia Internasional Indonesia, Jakarta, Indonesia

Aivyanca A, Institut Bio Scientia Internasional Indonesia

Department of Biotechnology, Institut Bio Scientia Internasional Indonesia, Jakarta, Indonesia

Charlivo Mikaichi Dungus, Institut Bio Scientia Internasional Indonesia

Department of Biotechnology, Institut Bio Scientia Internasional Indonesia, Jakarta, Indonesia

Jason Jonathan Hamid, Institut Bio Scientia Internasional Indonesia

Department of Biotechnology, Institut Bio Scientia Internasional Indonesia, Jakarta, Indonesia

Katherine Katherine, Institut Bio Scientia Internasional Indonesia

Department of Biotechnology, Institut Bio Scientia Internasional Indonesia, Jakarta, Indonesia


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How to Cite
Josefano, R., A, A., Dungus, C., Hamid, J., & Katherine, K. (2023). Bioprocessing of mAb (Monoclonal Antibodies) using Chinese Hamster Ovary (CHO) Cells: A Review. Indonesian Journal of Life Sciences, 5(01), 58-77.
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